The Police Divisions that border 53 Division are:

• 13 Division to the west,
• 54 Division to the east,
• 52 Division to the south
• 32 Division to the north.

The station is open 24 hours per day, and is located at 75 Eglinton Avenue West in the City of Toronto. This is just west of the Yonge/Eglinton subway station. There is limited public parking at the station parking lot located south of the building, off Duplex Avenue. Public transportation also provides easy direct access to the station. If arriving by subway, exit at the Yonge/Eglinton subway station, and walk west one block on Eglinton Ave W to Duplex Ave and the station is immediately in front of you on the south west corner.